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Ibrahim, Rana M., Fayez, Shaimaa, Eltanany, Basma M., Abu-Elghait, Mohammed, El-Demerdash, Amr 
ORCID: https://orcid.org/0000-0001-6459-2955, Badawy, Mona Shaban E. M., Pont, Laura, Benavente, Fernando and Saber, Fatema R.
(2024)
Agro-byproduct valorization of radish and turnip leaves and roots as new sources of antibacterial and antivirulence agents through metabolomics and molecular networking.
Scientia Horticulturae, 328.
ISSN 0304-4238
Abstract
In recent decades, the concept of sustainability has gained significant popularity, with a great emphasis on revalorizing agro-byproducts as potential sources of bioactive compounds. Radish and turnip are economically significant crops that are widely produced across the world for direct consumption or oil and condiment production. Additionally, they are also frequently used in traditional medicine to cure bacterial infections, becoming a potential source of antimicrobial and antivirulence agents to combat microbial resistance. Here, a comprehensive metabolite fingerprinting was adopted to characterize the leaves and roots of three varieties of radish (Daikon, white icicle, and red radish) and turnip concerning their antibacterial and antivirulence activities. A total of 120 metabolites from various classes were annotated using liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry (LC-QTOF-MS/MS) and molecular networking. Multivariate data analysis revealed that metabolites distribution and abundance differed between samples, with glucosinolates, flavonoids, phenolic, and fatty acids being the most significantly differential metabolites. Whereas daikon and turnip leaves were enriched in flavonoids and glucosinolates, red radish and turnip leaves were high in phenolic acids, and red radish roots were high in fatty acids. All extracts exhibited significant antibacterial activity against K. pneumoniae and P. aeruginosa. Daikon and turnip leaves prevented biofilm formation in K. pneumoniae by 99.7 % and 74.2 %, respectively, whereas white icicle, turnip, and daikon roots inhibited biofilm formation in P. aeruginosa by 69.7 %, 67.0 %, and 62.2 %, respectively. The roots of white icicle and red radish reduced P. aeruginosa swarming motility by 83.1 % and 83.3 %, respectively, whereas the roots of white icicle, daikon, and red radish reduced its swimming motility by 84.4 %, 82.5 %, and 82.4 %, respectively. Additionally, the leaves of white icicle radish inhibited pyocyanin production in P. aeruginosa by 76.8 %. Pearson's correlation analyses revealed that the bioactivities were linked to various phenolic and sulfur compounds. The current study is the first to combine metabolomics, molecular networking, and chemometrics approaches to unveil metabolome heterogeneity among radish and turnip leaves and roots, which could be derived from agro-byproducts to revalorize while validating their potential as promising sources of antivirulence phytochemicals.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Basma M. Eltanany would like to thank the Egyptian Ministry of Higher Education for financing her postdoctoral research stay at the Bioanalysis group of the University of Barcelona (UB), Barcelona, Spain. The Bioanalysis group of the UB is part of the INSA-UB Maria de Maeztu Unit of Excellence (Grant CEX2021-001234-M) funded by MICIN/AEI/FEDER, UE. |
| Uncontrolled Keywords: | antibacterial,antivirulence,metabolomics,molecular networking,radish,turnip,horticulture ,/dk/atira/pure/subjectarea/asjc/1100/1108 |
| Faculty \ School: | Faculty of Science > School of Pharmacy (former - to 2024) |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 12 Aug 2024 17:30 |
| Last Modified: | 25 Sep 2024 17:58 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/96203 |
| DOI: | 10.1016/j.scienta.2024.112924 |
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