Interaction between the origin recognition complex and the replication licensing system in Xenopus

Rowles, Alison, Chong, James P. J., Brown, Lamorna, Howell, Mike, Evan, Gerard I. and Blow, J. Julian ORCID: https://orcid.org/0000-0002-9524-5849 (1996) Interaction between the origin recognition complex and the replication licensing system in Xenopus. Cell, 87 (2). pp. 287-296. ISSN 0092-8674

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Abstract

The origin recognition complex (ORC) binds to origins of replication in budding yeast. We have cloned a Xenopus homolog of the largest ORC polypeptide (XORC1). Immunodepletion of XOrc1 from Xenopus egg extracts blocks the initiation of DNA replication. We have purified Xenopus ORC, consisting of a protein complex similar to yeast ORC. In Xenopus egg extracts, ORC associates with chromatin throughout G1 and S phases. RLF-M, a component of the replication licensing system, also associates with chromatin early in the cell cycle but dissociates during S phase. We show that the assembly of RLF-M onto chromatin is dependent on the presence of chromatin-bound ORC, leading to sequential assembly of initiation proteins onto replication origins during the cell cycle.

Item Type:	Article
Faculty \ School:
Depositing User:	LivePure Connector
Date Deposited:	11 Jun 2024 10:31
Last Modified:	25 Sep 2024 17:52
URI:	https://ueaeprints.uea.ac.uk/id/eprint/95565
DOI:	10.1016/S0092-8674(00)81346-X

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