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Mahbubani, H. M., Paull, T., Elder, J. K. and Blow, J. J. 
ORCID: https://orcid.org/0000-0002-9524-5849
(1992)
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
Nucleic Acids Research, 20 (7).
pp. 1457-1462.
ISSN 0305-1048
Abstract
Cell-free extracts of Xenopus eggs will replicate plasmid DNA molecules under normal cell cycle control. We have used the neutral/neutral 2-D gel technique to map the sites at which DNA replication initiates in this system. Three different plasmids were studied: one containing the Xenopus rDNA repeat, one containing single copy Xenopus genomic DNA, and another containing the yeast 2 microns replication origin. 2-D gel profiles show that many potential sites of initiation are present on each plasmid, and are randomly situated at the level of resolution of this technique (500-1000 bp). Despite the abundance of sites capable of supporting the initiation of replication, pulse-chase experiments suggest that only a single randomly situated initiation event occurs on each DNA molecule. Once initiation has taken place, conventional replication forks appear to move away from this site at a rate of about 10nt/second, similar to the rate observed in vivo.
| Item Type: | Article |
|---|---|
| Faculty \ School: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 11 Jun 2024 10:30 |
| Last Modified: | 25 Sep 2024 17:52 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/95557 |
| DOI: | 10.1093/nar/20.7.1457 |
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