Clusters, factories and domains: The complex structure of S-phase comes into focus

Gillespie, Peter J. and Blow, Julian (2010) Clusters, factories and domains: The complex structure of S-phase comes into focus. Cell Cycle, 9 (16). pp. 3238-3246. ISSN 1538-4101

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Abstract

During S-phase of the cell cycle, chromosomal DNA is replicated according to a complex replication timing program, with megabase-sized domains replicating at different times. DNA fibre analysis reveals that clusters of adjacent replication origins fire near-synchronously. Analysis of replicating cells by light microscopy shows that DNA synthesis occurs in discrete foci or factories. The relationship between timing domains, origin clusters and replication foci is currently unclear. Recent work, using a hybrid Xenopus/hamster replication system, has shown that when CDK levels are manipulated during S-phase the activation of replication factories can be uncoupled from progression through the replication timing program. Here, we use data from this hybrid system to investigate potential relationships between timing domains, origin clusters and replication foci. We suggest that each timing domain typically comprises several replicon clusters, which are usually processed sequentially by replication factories. We discuss how replication might be regulated at different levels to create this complex organisation and the potential involvement of CDKs in this process.

Item Type:	Article
Uncontrolled Keywords:	dna replication,cdks,s-phase,replication timing,replicon clusters,replication foci,replication factories,chromosome domains,replication timing domains,cell-cycle regulation,dna-replication,chromosomal domains,replicon clusters,dormant origins,excess mcm2-7,g1 phase,xenopus,organization,chromatin
Faculty \ School:
Depositing User:	LivePure Connector
Date Deposited:	10 Jun 2024 10:31
Last Modified:	10 Jun 2024 10:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/95438
DOI:	10.4161/cc.9.16.12644

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