The phosphorylation landscape of infection-related development by the rice blast fungus

Cruz-Mireles, Neftaly, Osés-Ruiz, Miriam, Derbyshire, Paul, Jégousse, Clara, Ryder, Lauren S., Bautista, Mark Jave A., Eseola, Alice, Sklenar, Jan, Tang, Bozeng, Yan, Xia, Ma, Weibin, Findlay, Kim C., Were, Vincent, MacLean, Dan, Talbot, Nicholas J. ORCID: https://orcid.org/0000-0001-6434-7757 and Menke, Frank L. H. ORCID: https://orcid.org/0000-0003-2490-4824 (2024) The phosphorylation landscape of infection-related development by the rice blast fungus. Cell, 187 (10). 2557-2573.e18. ISSN 0092-8674

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Abstract

Many of the world’s most devastating crop diseases are caused by fungal pathogens that elaborate specialized infection structures to invade plant tissue. Here, we present a quantitative mass-spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins following germination on a hydrophobic surface, revealing major re-wiring of phosphorylation-based signaling cascades during appressorium development. Comparing phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring (PRM) to identify phosphoproteins regulated by the fungal Pmk1 MAPK that controls plant infection by M. oryzae. We define 32 substrates of Pmk1 and show that Pmk1-dependent phosphorylation of regulator Vts1 is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for the control of plant diseases.

Item Type:	Article
Additional Information:	Funding information: This work was supported by grants to N.J.T. from the Gatsby Charitable Foundation and Biotechnology and Biological Sciences Research Council (BBSRC) BBS/E/J/000PR9797, a BBSRC grant to N.J.T. and F.L.H.M. (BB/V016342/1), and the BBSRC core capability grant to Bioimaging facilities at the JIC. N.C.-M. was supported by the John Innes Foundation, the Sainsbury Laboratory Rotation Programme PhD Studentship, and the Consejo Nacional de Humanidades, Ciencia y Tecnologías (CONAHCYT). M.O.-R. was supported by the Spanish Ministry of Science and Innovation Grant, PID2020-120188RJ-I00, financed by MICIN/AEI. M.O.R. is a recipient of the Ramon y Cajal grant RYC2021-032146-I, funded by MCIN/AEI/10.13039/5011000110033, “European Union Next Generation EU/PRTR,” and the European Union (HORIZON-ERC2021-101076478).
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Depositing User:	LivePure Connector
Date Deposited:	14 May 2024 11:31
Last Modified:	18 Dec 2024 01:37
URI:	https://ueaeprints.uea.ac.uk/id/eprint/95192
DOI:	10.1016/j.cell.2024.04.007

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