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César-Razquin, Adrián, Casacuberta, Josep, Dalmay, Tamas 
ORCID: https://orcid.org/0000-0003-1492-5429, Federici, Silvia, Jacchia, Sara, Kagkli, Dafni Maria, Moxon, Simon ORCID: https://orcid.org/0000-0003-4644-1816 and Papadopoulou, Nikoletta
and
European Food Safety Authority (EFSA)
(2024)
Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants.
EFSA Journal, 22 (4).
ISSN 1831-4732
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Abstract
As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. This Technical Note to the applicants puts together requirements and recommendations for the quality assessment of the methodology, analysis and reporting when DNA sequencing is used for the molecular characterisation of GM plants. In particular, it applies to the use of Sanger sequencing and next-generation sequencing for the characterisation of the inserted genetic material and its flanking regions at each insertion site, the determination of the copy number of all detectable inserts and the analysis of the genetic stability of the inserts. This updated document replaces the EFSA 2018 Technical Note and reflects the current knowledge in scientific-technical methods for generating and verifying, in a standardised manner, DNA sequencing data in the context of RA of GM plants. It does not take into consideration the verification and validation of the detection method which remains under the remit of the Joint Research Centre (JRC).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | dna sequencing,genetic stability,genetically modified organisms,molecular characterisation,next-generation sequencing,ngs,risk assessment,food science,animal science and zoology,veterinary (miscalleneous),plant science,parasitology,microbiology,sdg 2 - zero hunger ,/dk/atira/pure/subjectarea/asjc/1100/1106 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 19 Apr 2024 11:30 |
| Last Modified: | 30 Sep 2024 00:25 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/94974 |
| DOI: | 10.2903/j.efsa.2024.8744 |
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