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Carroll, Joseph J., Sprigg, Colleen, Chilvers, Graham, Delso, Ignacio 
ORCID: https://orcid.org/0000-0001-8355-2289, Barker, Megan, Cox, Filipa, Johnson, David and Brearley, Charles A. ORCID: https://orcid.org/0000-0001-6179-9109
(2024)
LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine‐scale mapping of inositol phosphate distribution.
Methods in Ecology and Evolution, 15 (3).
pp. 530-543.
ISSN 2041-210X
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Abstract
1. Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. 3. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. 3. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. 4. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.
| Item Type: | Article |
|---|---|
| Additional Information: | Research Funding: Natural Environment Research Council. Grant Number: NE/W000350/1; Biotechnology and Biological Sciences Research Council. Grant Number: BB/M011216/1 |
| Uncontrolled Keywords: | lc-icp-ms,inositol phosphate isomers,nitrogen fertilization,phosphorus speciation,phytate,soil 31p nmr,ecology, evolution, behavior and systematics,ecological modelling ,/dk/atira/pure/subjectarea/asjc/1100/1105 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science Faculty of Science > School of Pharmacy |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 04 Mar 2024 18:28 |
| Last Modified: | 09 Apr 2024 03:36 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/94453 |
| DOI: | 10.1111/2041-210X.14292 |
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