LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine‐scale mapping of inositol phosphate distribution

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Carroll, Joseph J., Sprigg, Colleen, Chilvers, Graham, Delso, Ignacio, Barker, Megan, Cox, Filipa, Johnson, David and Brearley, Charles A. (2024) LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine‐scale mapping of inositol phosphate distribution. Methods in Ecology and Evolution, 15 (3). pp. 530-543. ISSN 2041-210X

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Abstract

1. Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. 3. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. 3. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. 4. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.

Item Type:	Article
Additional Information:	Research Funding: Natural Environment Research Council. Grant Number: NE/W000350/1; Biotechnology and Biological Sciences Research Council. Grant Number: BB/M011216/1
Uncontrolled Keywords:	lc-icp-ms,inositol phosphate isomers,nitrogen fertilization,phosphorus speciation,phytate,soil 31p nmr,ecology, evolution, behavior and systematics,ecological modelling ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Molecular Microbiology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	04 Mar 2024 18:28
Last Modified:	06 Feb 2025 11:48
URI:	https://ueaeprints.uea.ac.uk/id/eprint/94453
DOI:	10.1111/2041-210X.14292

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