A transcriptional and regulatory map of mouse somite maturation

Ibarra-Soria, Ximena, Thierion, Elodie, Mok, Gi Fay ORCID: https://orcid.org/0000-0002-5202-9062, Münsterberg, Andrea E. ORCID: https://orcid.org/0000-0002-4577-4240, Odom, Duncan T. and Marioni, John C. (2023) A transcriptional and regulatory map of mouse somite maturation. Developmental Cell, 58 (19). 1983-1995.e7. ISSN 1534-5807

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Abstract

The mammalian body plan is shaped by rhythmic segmentation of mesoderm into somites, which are transient embryonic structures that form down each side of the neural tube. We have analyzed the genome-wide transcriptional and chromatin dynamics occurring within nascent somites, from early inception of somitogenesis to the latest stages of body plan establishment. We created matched gene expression and open chromatin maps for the three leading pairs of somites at six time points during mouse embryonic development. We show that the rate of somite differentiation accelerates as development progresses. We identified a conserved maturation program followed by all somites, but somites from more developed embryos concomitantly switch on differentiation programs from derivative cell lineages soon after segmentation. Integrated analysis of the somitic transcriptional and chromatin activities identified opposing regulatory modules controlling the onset of differentiation. Our results provide a powerful, high-resolution view of the molecular genetics underlying somitic development in mammals.

Item Type: Article
Additional Information: Funding Information: The authors would like to thank Aaron Lun for valuable suggestions on the analytical strategy, Pascale Gilardi-Hebenstreit for insightful comments on the manuscript, and CRUK Cambridge Institute Bioinformatics and Biological Resources Unit Core Facilities. This work was supported by Cancer Research UK (to E.T. and D.T.O.: 20412 and 22398 ), the Wellcome Trust (to X.I.-S.: 108438/Z/15 to J.C.M. and 202878/Z/16/Z to D.T.O.), the European Molecular Biology Laboratory (to J.C.M.), the European Research Council (to D.T.O.: 615584 and 788937 ), and Helmholtz NCT funding (D.T.O.: DKFZ Abteiling B270).
Uncontrolled Keywords: atac-seq,chromatin,gene regulation,mouse development,rna-seq,somite maturation,somites,transcription,molecular biology,biochemistry, genetics and molecular biology(all),developmental biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 24 Jan 2024 01:35
Last Modified: 24 Jan 2024 01:35
URI: https://ueaeprints.uea.ac.uk/id/eprint/94236
DOI: 10.1016/j.devcel.2023.07.003

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