The effect of protein supplementation on markers of bone metabolism following repeated whole-body resistance exercise in men and women: A randomised controlled trial

Greeves, Julie, Mallison, J. E., Wardle, Sophie L., Atherton, P, Greenhaff, Paul L., Wilkinson, D. L., Tang, Jonathan C. Y., Fraser, William and O'Leary, Thomas J. (2023) The effect of protein supplementation on markers of bone metabolism following repeated whole-body resistance exercise in men and women: A randomised controlled trial. In: American Society for Bone and Mineral Research, 2023-10-13 - 2023-10-16, Vancouver Convention & Exhibition Centre.

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Abstract

This study examined acute, dose-dependent effects of protein supplementation on markers of bone metabolism in young healthy adults following two repeated bouts of whole-body resistance exercise in a randomised controlled trial. Resistance-trained men (n = 24) and women (n = 24) aged 18 to 35 y performed two within-day whole-body resistance sessions in the morning (Ex 1) and afternoon (Ex 2), separated by 4 h. Exercises comprised 3 × 8 repetitions at 75% 1 rep max (2 min rest between sets) involving latissimus dorsi pull down, single-leg press, and chest press. Participants (8 men and 8 women per group) were randomly assigned to drink either 15 g, 30 g or 60 g whey protein immediately after each exercise session. Resting venous blood samples were drawn after an overnight fast at 0 h (before Ex 1), 4 h (before Ex 2), 8 h, and 24 h. Samples were analysed for CTX, P1NP, BALP, OC, RANKL, OPG, Sclerostin (SOST), PTH, adjusted-calcium. The dose-dependent effects of protein supplementation were analysed using Linear Mixed Effects Models. p values were adjusted using the Holm-Bonferroni method and significance was accepted at p < 0.05. A significant main effect of time was shown for CTX, P1NP, OC, sRANKL, OPG, and PTH. CTX decreased between 0 h and 4 h, and 0 h and 8 h (p < 0.001). P1NP decreased between 0 h and 24 h (p = 0.008). OC decreased between 0 h and 4 h, and 0 h and 8 h (p < 0.001). sRANKL decreased between 0 h and 4 h, 0 h and 8 h, and 0 h and 24 h (p < 0.001). OPG decreased between 0 h and 8 h (p = 0.008). PTH decreased between 0 h and 8 h (p=0.003). A group * interaction was shown for OC (p=0.015), and a group effect for SOST (15 g and 30 g) (p=0.037). Protein supplementation ingested immediately after two repeated same-day resistance exercise sessions did not increase markers of bone formation at any dose. A decrease in markers of bone resorption, molecular signalling makers of bone metabolism, and PTH are likely due to responses in circadian variation.

Item Type:	Conference or Workshop Item (Other)
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	LivePure Connector
Date Deposited:	22 Dec 2023 02:24
Last Modified:	06 Feb 2025 01:20
URI:	https://ueaeprints.uea.ac.uk/id/eprint/94038
DOI:

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