Triazine probes target ascorbate peroxidases in plants

Morimoto, Kyoko, Cole, Kyle S., Kourelis, Jiorgos ORCID: https://orcid.org/0000-0002-9007-1333, Witt, Collin H., Brown, Daniel, Krahn, Daniel, Stegmann, Monika, Kaschani, Farnusch, Kaiser, Markus, Burton, Jonathan, Mohammed, Shabaz, Yamaguchi-Shinozaki, Kazuko, Weerapana, Eranthie and van der Hoorn, Renier A. L. (2019) Triazine probes target ascorbate peroxidases in plants. Plant Physiology, 180 (4). pp. 1848-1859. ISSN 0032-0889

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Abstract

Though they are rare in nature, anthropogenic 1,3,5-triazines have been used in herbicides as chemically stable scaffolds. Here, we show that small 1,3,5-triazines selectively target ascorbate peroxidases (APXs) in Arabidopsis (Arabidopsis thaliana), tomato (Solanum lycopersicum), rice (Oryza sativa), maize (Zea mays), liverwort (Marchantia polymorpha), and other plant species. The alkyne-tagged 2-chloro-4-methyl-1,3,5-triazine probe KSC-3 selectively binds APX enzymes, both in crude extracts and in living cells. KSC-3 blocks APX activity, thereby reducing photosynthetic activity under moderate light stress, even in apx1 mutant plants. This suggests that APX enzymes in addition to APX1 protect the photosystem against reactive oxygen species. Profiling APX1 with KCS-3 revealed that the catabolic products of atrazine (a 1,3,5-triazine herbicide), which are common soil pollutants, also target APX1. Thus, KSC-3 is a powerful chemical probe to study APX enzymes in the plant kingdom.

Item Type:	Article
Additional Information:	Funding Information: This work was supported by the Strategic Young Investigator Overseas Visit Program for Enhanced Brain Circulation of the Japanese Society for the Promotion of Science (JSPS grant to K.M.), the John Fell Fund (to K.M.), the European Research Council (ERC Consolidator grant 616449 “GreenProteases” to K.M., J.K., and R.H.), the Clarendon Fund (to J.K.), the Biotechnology and Biological Sciences Research Council (BBSRC grants BB/R017913/1 and BB/S003193/ 1 to R.H.), and the National Institutes of Health (NIH grants 1R01GM117004 and 1R01GM118431-01A1 to E.W.).
Uncontrolled Keywords:	physiology,genetics,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	07 Dec 2023 01:46
Last Modified:	01 Feb 2024 03:07
URI:	https://ueaeprints.uea.ac.uk/id/eprint/93897
DOI:	10.1104/pp.19.00481

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