Genomics of Acinetobacter baumannii iron uptake

Artuso, Irene, Poddar, Harsh, Evans, Benjamin A. ORCID: https://orcid.org/0000-0001-6849-9758 and Visca, Paolo (2023) Genomics of Acinetobacter baumannii iron uptake. Microbial Genomics, 9 (8). ISSN 2057-5858

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Abstract

Iron is essential for growth in most bacteria due to its redox activity and its role in essential metabolic reactions; it is a cofactor for many bacterial enzymes. The bacterium Acinetobacter baumannii is a multidrug-resistant nosocomial pathogen. A. baumannii responds to low iron availability imposed by the host through the exploitation of multiple iron-acquisition strategies, which are likely to deliver iron to the cell under a variety of environmental conditions, including human and animal infection. To date, six different gene clusters for active iron uptake have been described in A. baumannii , encoding protein systems involved in (i) ferrous iron uptake (feo); (ii) haem uptake (hemT and hemO); and (iii) synthesis and transport of the baumannoferrin(s) (bfn), acinetobactin (bas/bau) and fimsbactin(s) (fbs) siderophores. Here we describe the structure, distribution and phylogeny of iron-uptake gene clusters among >1000 genotypically diverse A. baumannii isolates, showing that feo, hemT, bfn and bas/bau clusters are very prevalent across the dataset, whereas the additional haem-uptake system hemO is only present in a portion of the dataset and the fbs gene cluster is very rare. Since the expression of multiple iron-uptake clusters can be linked to virulence, the presence of the additional haem-uptake system hemO may have contributed to the success of some A. baumannii clones.

Item Type:	Article
Additional Information:	Funding Information: This work was funded by grants from Rome Technopole Foundation (Spoke 1, CUP F83B22000040006), the National Biodiversity Future Centre (Spoke 3, CUP F83C22000730006) Grant no. A0375-2020-36558 GAVAP from Regione Lazio and MUR-PRIN 2017, grant prot. 20177J5Y3P to P.V. I.A. thanks the Federation of European Microbiological Societies (FEMS) for supporting her research at Norwich Medical School with a Research and Training grant.
Uncontrolled Keywords:	acinetobacter baumannii,haem,iron uptake,phylogeny,sequence type,siderophore,epidemiology,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2700/2713
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	18 Aug 2023 09:32
Last Modified:	19 Oct 2023 03:37
URI:	https://ueaeprints.uea.ac.uk/id/eprint/92882
DOI:	10.1099/mgen.0.001080

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