Design of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agents

Shah, Anokhi, Taylor, Michael J., Molinaro, Giulia, Anbu, Sellamuthu ORCID: https://orcid.org/0000-0002-7176-330X, Verdu, Margaux, Jennings, Lucy, Mikulska, Iuliia, Díaz-Moreno, Sofía, EL Mkami, Hassane, Smith, Graham M., Britton, Melanie M., Lovett, Janet E. and Peacock, Anna F. A. (2023) Design of the elusive proteinaceous oxygen donor copper site suggests a promising future for copper for MRI contrast agents. Proceedings of the National Academy of Sciences of the United States of America, 120 (27). ISSN 0027-8424

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Abstract

We report the preparation and spectroscopic characterization of a highly elusive copper site bound exclusively to oxygen donor atoms within a protein scaffold. Despite copper generally being considered unsuitable for use in MRI contrast agents, which in the clinic are largely Gd(III) based, the designed copper coiled coil displays relaxivity values equal to, or superior than, those of the Gd(III) analog at clinical field strengths. The creation of this new-to-biology proteinaceous CuOx-binding site demonstrates the power of the de novo peptide design approach to access chemistry for abiological applications, such as for the development of MRI contrast agents.

Item Type:	Article
Uncontrolled Keywords:	mri,bioinorganic chemistry,coiled coil,contrast agents,protein design
Faculty \ School:	Faculty of Science > School of Chemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	30 Jun 2023 15:30
Last Modified:	30 Jan 2024 03:37
URI:	https://ueaeprints.uea.ac.uk/id/eprint/92549
DOI:	10.1073/pnas.2219036120

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