Investigating DNA’s mysterious structures: development of probes to target the i-Motif forming region of the MYC promoter

Rosonovski, Summer (2021) Investigating DNA’s mysterious structures: development of probes to target the i-Motif forming region of the MYC promoter. Doctoral thesis, University of East Anglia.

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Abstract

Chapter 1 is a general introduction to DNA and the alternative secondary structures that it forms, leading into an introduction to MYC the oncogene and techniques used to determine probes that bind to the i-motif that forms in the MYC promoter region.

Chapter 2 explores the interactions of small molecules with the MYC i-motif DNA using a high-throughput biophysical screen with a library of 1596 compounds. The results are validated by further biophysical techniques with the most promising compounds continued into the next stages of development finishing with the beginning of cellular studies.

Chapter 3 explores the interactions of peptides with the MYC i-motif DNA using phage display with different phage libraries contain 109 different peptide sequences. The first section discusses method development of the phage display technique to achieve optimal conditions. The second uses biophysical techniques to demonstrate peptides binding to the MYC i-motif.

Chapter 4 discusses Chapters 2 and 3 in the context of the current literature and explores the future work that this project could take.

Chapter 5 discusses the experimental procedures used in Chapters 2 and 3.

Item Type:	Thesis (Doctoral)
Faculty \ School:	Faculty of Science > School of Pharmacy
Depositing User:	Nicola Veasy
Date Deposited:	23 Mar 2023 12:39
Last Modified:	23 Mar 2023 12:39
URI:	https://ueaeprints.uea.ac.uk/id/eprint/91677
DOI:

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