Complex scaffold remodeling in plant triterpene biosynthesis

Pena, Ricardo De La, Hodgson, Hannah, Liu, Chun-Ting, Stephenson, Michael J ORCID:, Martin, Azahara Carmen, Owen, Charlotte, Harkess, Alex, Leebens-Mack, James, Jimenez, Luis, Osbourn, Anne and Sattely, Elizabeth (2023) Complex scaffold remodeling in plant triterpene biosynthesis. Science, 379 (6630). pp. 361-368. ISSN 0036-8075

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Triterpenes with complex scaffold modifications are widespread in the plant kingdom. Limonoids are an exemplary family that are responsible for the bitter taste in citrus (e.g., limonin) and the active constituents of neem oil, a widely used bioinsecticide (e.g., azadirachtin). Despite the commercial value of limonoids, a complete biosynthetic route has not been described. We report the discovery of 22 enzymes, including a pair of neofunctionalized sterol isomerases, that catalyze 12 distinct reactions in the total biosynthesis of kihadalactone A and azadirone, products that bear the signature limonoid furan. These results enable access to valuable limonoids and provide a template for discovery and reconstitution of triterpene biosynthetic pathways in plants that require multiple skeletal rearrangements and oxidations.

Item Type: Article
Uncontrolled Keywords: biosynthesis,triterpenoids,limonoids,transient expression
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
Depositing User: LivePure Connector
Date Deposited: 28 Feb 2023 10:30
Last Modified: 28 Feb 2023 10:30
DOI: 10.1126/science.adf1017


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