Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

Ribeiro, A. P. C., Anbu, S. ORCID: https://orcid.org/0000-0002-7176-330X, Alegria, E. C. B. A., Fernandes, A. R., Baptista, P. V., Mendes, R., Matias, A. S., Mendes, M., Guedes da Silva, M. F. C. and Pombeiro, A. J. L. (2018) Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine and Pharmacotherapy, 101. pp. 137-144. ISSN 1950-6007

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Abstract

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV–vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	21 Feb 2023 09:30
Last Modified:	30 Jan 2024 03:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/91236
DOI:	10.1016/j.biopha.2018.02.069

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