Authentication of saffron using 60 MHz 1H NMR spectroscopy

Gunning, Yvonne, Davies, Kate S. and Kemsley, E. Kate ORCID: (2023) Authentication of saffron using 60 MHz 1H NMR spectroscopy. Food Chemistry, 404 (Part B). ISSN 0308-8146

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60 MHz proton NMR spectroscopy was used to analyse extracts from saffron spice and a range of potential adulterants and mixtures. Using a simple extraction procedure, good quality spectra were obtained which contain peaks from the characteristic metabolites picrocrocin and crocins, fatty acids and kaempferol. The spectra of samples from trusted suppliers were used to train one-class classification models by SIMCA, nearest neighbour and isolation forest methods. Applying these to spectra of saffron samples purchased from the online marketplace, it was found that 7 out of 33 samples were highly anomalous. From comparison with the spectra of known mixtures and confirmatory spectral analysis using 600 MHz NMR, it is probable that these contain considerable amounts of undisclosed foreign matter.

Item Type: Article
Additional Information: Funding Information: The authors gratefully acknowledge the support of the Biotechnology and Biological Sciences Research Council (BBSRC); this research was funded by the BBSRC Core Capability Grant number BB/CCG1860/1. We would also like to thank our colleagues in the Core Science Resources group at Quadram Institute Bioscience for their help in cultivating saffron plants for use in this work.
Uncontrolled Keywords: adulteration,anomaly,norwich,benchtop,nmr,outlier,saffron,spectroscopy,analytical chemistry,food science ,/dk/atira/pure/subjectarea/asjc/1600/1602
Faculty \ School: Faculty of Science > School of Chemistry
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Depositing User: LivePure Connector
Date Deposited: 07 Feb 2023 10:33
Last Modified: 17 Feb 2023 10:30
DOI: 10.1016/j.foodchem.2022.134649

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