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Malec, Katarzyna, Monaco, Serena, Delso, Ignacio 
ORCID: https://orcid.org/0000-0001-8355-2289, Nestorowicz, Justyna, Kozakiewicz-Latała, Marta, Karolewicz, Bożena, Khimyak, Yaroslav ORCID: https://orcid.org/0000-0003-0424-4128, Angulo, Jesus ORCID: https://orcid.org/0000-0001-7250-5639 and Nartowski, Karol
(2023)
Unravelling the mechanisms of drugs partitioning phenomena in micellar systems via NMR spectroscopy.
Journal of Colloid and Interface Science, 638.
pp. 135-148.
ISSN 0021-9797
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Abstract
Despite extensive use of micelles in materials and colloidal science, their supramolecular organization as well as host-guest interactions within these dynamic assemblies are poorly understood. Small guest molecules in the presence of micelles undergo constant exchange between a micellar aggregate and the surrounding solution, posing a considerable challenge for their molecular level characterisation. In this work we reveal the interaction maps between small guest molecules and surfactants forming micelles via novel applications of NMR techniques supported with state-of-the-art analytical methods used in colloidal science. Model micelles composed of structurally distinct surfactants (block non-ionic polymer Pluronic® F-127, non-ionic surfactant Tween 20 or Tween 80 and ionic surfactant SLS, sodium lauryl sulphate) were selected and loaded with model small molecules of biochemical relevance (i.e. the drugs fluconazole, FLU or indomethacin, IMC) known to have different partition coefficients. Molecular level organization of FLU or IMC within hydrophilic and hydrophobic domains of micellar aggregates was established using the combination of NMR methods (1D 1H NMR, 1D 19F NMR, 2D 1H-1H NOESY and 2D 1H-19F HOESY, and the multifrequency-STD NMR) and corroborated with molecular dynamics (MD) simulations. This is the first application of multifrequency-STD NMR to colloidal systems, enabling us to elucidate intricately detailed patterns of drug/micelle interactions in a single NMR experiment within minutes. Importantly, our results indicate that flexible surfactants, such as block copolymers and polysorbates, form micellar aggregates with a surface composed of both hydrophilic and hydrophobic domains and do not follow the classical core-shell model of the micelle. We propose that the magnitude of the changes in 1H chemical shifts corroborated with interaction maps obtained from DEEP-STD NMR and 2D NMR experiments can be used as an indicator of the strength of the guest-surfactant interactions. This NMR toolbox can be adopted for the analysis of the broad range of colloidal host-guest systems from soft materials to biological systems.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: KM would like to acknowledge funding from National Science Centre in Poland via the doctoral scholarship (Doctoral Scholarship ETIUDA: 2019/32/T/NZ7/00246). The funding for materials was obtained from Ministry of Science and Higher Education in Poland (internal number at WMU: STM.D190.18.019). The authors are grateful to UEA NMR platforms as part of UEA Faculty of Science facilities. The research presented in this work was carried out using the High Performance Computing Cluster supported by the Research and Specialist Computing Support service at the University of East Anglia. |
| Uncontrolled Keywords: | electronic, optical and magnetic materials,biomaterials,surfaces, coatings and films,colloid and surface chemistry ,/dk/atira/pure/subjectarea/asjc/2500/2504 |
| Faculty \ School: | Faculty of Science > School of Pharmacy |
| UEA Research Groups: | Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Jan 2023 11:30 |
| Last Modified: | 20 Jan 2024 01:38 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/90744 |
| DOI: | 10.1016/j.jcis.2023.01.063 |
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