Responses of Salmonella biofilms to oxidizing biocides: Evidence of spatial clustering

Guest, Kerry, Whalley, Thomas, Maillard, Jean‐Yves, Artemiou, Andreas, Szomolay, Barbara and Webber, Mark A. (2022) Responses of Salmonella biofilms to oxidizing biocides: Evidence of spatial clustering. Environmental Microbiology, 24 (12). pp. 6426-6438. ISSN 1462-2912

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Abstract

The spatial organization of biofilm bacterial communities can be influenced by several factors, including growth conditions and challenge with antimicrobials. Differential survival of clusters of cells within biofilms has been observed. In this work, we present a variety of methods to identify, quantify and statistically analyse clusters of live cells from images of two Salmonella strains with differential biofilm forming capacity exposed to three oxidizing biocides. With a support vector machine approach, we showed spatial separation between the two strains, and, using statistical testing and high-performance computing (HPC), we determined conditions which possess an inherent cluster structure. Our results indicate that there is a relationship between biocide potency and inherent biofilm formation capacity with the tendency to select for spatial clusters of survivors. There was no relationship between positions of clusters of live or dead cells within stressed biofilms. This work identifies an approach to robustly quantify clusters of physiologically distinct cells within biofilms and suggests work to understand how clusters form and survive is needed.

Item Type:	Article
Additional Information:	Funding Information: Work into biofilms by MAW was supported by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10349.
Uncontrolled Keywords:	microbiology,ecology, evolution, behavior and systematics ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	http://www.scopus.com/inward/record.url?... https://onlinelibrary.wiley.com/doi/10.1...
Depositing User:	LivePure Connector
Date Deposited:	15 Dec 2022 03:59
Last Modified:	03 Jan 2023 09:34
URI:	https://ueaeprints.uea.ac.uk/id/eprint/90115
DOI:	10.1111/1462-2920.16263

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