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Ghany, Moataz Abd El, Barquist, Lars, Clare, Simon, Brandt, Cordelia, Mayho, Matthew, Joffré, Enrique, Sjöling, Åsa, Turner, A. Keith, Klena, John D., Kingsley, Robert A. 
ORCID: https://orcid.org/0000-0002-0194-6485, Hill-Cawthorne, Grant A., Dougan, Gordon and Pickard, Derek
(2021)
Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization.
Microbial Genomics, 7 (6).
ISSN 2057-5858
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Abstract
Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by The Wellcome Trust (G.D.) and Marie Bashir Institute and Sydney Medical School foundation (M.A. and G.A.H.). The work was also supported by an ESPRC Vaccine-Hub grant and the NIMR Cambridge BRC Antibiotic Resistance theme (G.D.) and the Swedish Research Council (Å.S.). R.A.K. was funded by the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10348. The funders had no role in study design, data collection and interpretation or the decision to submit the work for publication. |
| Uncontrolled Keywords: | i,etec,h10407,rnaseq,tradis,water survival and colonization,epidemiology,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2700/2713 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 04 Nov 2022 10:30 |
| Last Modified: | 07 Jun 2024 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/89614 |
| DOI: | 10.1099/MGEN.0.000554 |
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