Borner, Georg H. H., Rana, Amer A. ORCID: https://orcid.org/0000-0002-2330-4643, Forster, Rebecca, Harbour, Michael, Smith, James C. and Robinson, Margaret S.
(2007)
CVAK104 is a novel regulator of clathrin-mediated SNARE sorting.
Traffic, 8 (7).
pp. 893-903.
ISSN 1398-9219
Abstract
Clathrin-coated vesicles (CCVs) mediate transport between the plasma membrane, endosomes and the trans Golgi network. Using comparative proteomics, we have identified coated-vesicle-associated kinase of 104 kDa (CVAK104) as a candidate accessory protein for CCV-mediated trafficking. Here, we demonstrate that the protein colocalizes with clathrin and adaptor protein-1 (AP-1), and that it is associated with a transferrin-positive endosomal compartment. Consistent with these observations, clathrin as well as the cargo adaptors AP-1 and epsinR can be coimmunoprecipitated with CVAK104. Small interfering RNA (siRNA) knockdown of CVAK104 in HeLa cells results in selective loss of the SNARE proteins syntaxin 8 and vti1b from CCVs. Morpholino-mediated knockdown of CVAK104 in Xenopus tropicalis causes severe developmental defects, including a bent body axis and ventral oedema. Thus, CVAK104 is an evolutionarily conserved protein involved in SNARE sorting that is essential for normal embryonic development.
Item Type: | Article |
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Uncontrolled Keywords: | ap-1,ap-2,cvak104,epsinr,sirna,snare,xenopus,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1315 |
Faculty \ School: | Faculty of Science > School of Biological Sciences |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 01 Nov 2022 14:32 |
Last Modified: | 02 Nov 2022 13:30 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/89475 |
DOI: | 10.1111/j.1600-0854.2007.00576.x |
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