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Rhodes, Jack, Roman, Andra-Octavia, Bjornson, Marta, Brandt, Benjamin, Derbyshire, Paul, Wyler, Michele, Schmid, Marc W., Menke, Frank L. H. 
ORCID: https://orcid.org/0000-0003-2490-4824, Santiago, Julia and Zipfel, Cyril
(2022)
Perception of a conserved family of plant signalling peptides by the receptor kinase HSL3.
eLife, 11.
ISSN 2050-084X
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Abstract
Plant genomes encode hundreds of secreted peptides; however, relatively few have been characterised. We report here an uncharacterised, stress‐induced family of plant signalling peptides, which we call CTNIPs. Based on the role of the common co‐receptor BRASSINOSTEROID INSENSITIVE 1‐ASSOCIATED KINASE 1 (BAK1) in CTNIP‐induced responses, we identified in Arabidopsis thaliana the orphan receptor kinase HAESA‐LIKE 3 (HSL3) as the CTNIP receptor via a proteomics approach. CTNIP binding, ligand‐triggered complex formation with BAK1, and induced downstream responses all involve HSL3. Notably, the HSL3‐CTNIP signalling module is evolutionarily conserved amongst most extant angiosperms. The identification of this novel signalling module will further shed light on the diverse functions played by plant signalling peptides and will provide insights into receptor-ligand co‐evolution.
| Item Type: | Article |
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| Additional Information: | Funding Information: H2020 European Research Council (773153); H2020 European Research Council (716358); The Gatsby Charitable Foundation; Universität Zürich; Swiss National Science Foundation (31003A_182625 and 31003A_173101); Fondation philanthropique Famille Sandoz; H2020 Marie Skłodowska-Curie Actions (703954); Biotechnology and Biological Sciences Research Council (BB/P012574/1). Data availability: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029264 and 10.6019/PXD029264. The RNA-seq datasets generated and analysed in the current study have been deposited in the ArrayExpress database at EMBL-EBI (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-11093. The following data sets were generated: Rhodes J, Derbyshire P, Menke F (2022) PRIDE ID PXD029264. Perception of a conserved family of plant signaling peptides by the receptor kinase HSL3. http://www.ebi.ac.uk/pride/archive/projects/PXD029264. Rhodes J, Bjornson M (2022) ArrayExpress ID E-MTAB-11093. RNAseq of Arabidopsis seedlings WT or hsl3-1, in response to a 30-min treatment of 100 nM CTNIP4 relative to mock. https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11093 |
| Uncontrolled Keywords: | neuroscience(all),biochemistry, genetics and molecular biology(all),immunology and microbiology(all) ,/dk/atira/pure/subjectarea/asjc/2800 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Oct 2022 15:31 |
| Last Modified: | 24 Oct 2022 16:36 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/89308 |
| DOI: | 10.7554/eLife.74687 |
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