Gui, Xinmeng, Zhang, Peng, Wang, Dan, Ding, Zhan, Wu, Xian, Shi, Jinxia, Shen, Qian Hua, Xu, Yong Zhen, Ma, Wenbo and Qiao, Yongli (2022) Phytophthora effector PSR1 hijacks the host pre-mRNA splicing machinery to modulate small RNA biogenesis and plant immunity. The Plant Cell, 34 (9). pp. 3443-3459. ISSN 1040-4651
Full text not available from this repository. (Request a copy)Abstract
Phytophthora effector PSR1 suppresses small RNA (sRNA)-mediated immunity in plants, but the underlying mechanism remains unknown. Here, we show that Phytophthora suppressor of RNA silencing 1 (PSR1) contributes to the pathogenicity of Phytophthora sojae and specifically binds to three conserved C-terminal domains of the eukaryotic PSR1-Interacting Protein 1 (PINP1). PINP1 encodes PRP16, a core pre-mRNA splicing factor that unwinds RNA duplexes and binds to primary microRNA transcripts and general RNAs. Intriguingly, PSR1 decreased both RNA helicase and RNA-binding activity of PINP1, thereby dampening sRNA biogenesis and RNA metabolism. The PSR1-PINP1 interaction caused global changes in alternative splicing (AS). A total of 5,135 genes simultaneously exhibited mis-splicing in both PSR1-overexpressing and PINP1-silenced plants. AS upregulated many mRNA transcripts that had their introns retained. The high occurrence of intron retention in AS-induced transcripts significantly promoted Phytophthora pathogen infection in Nicotiana benthamiana, and this might be caused by the production of truncated proteins. Taken together, our findings reveal a key role for PINP1 in regulating sRNA biogenesis and plant immunity.
Item Type: | Article |
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Uncontrolled Keywords: | plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110 |
Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 13 Oct 2022 10:39 |
Last Modified: | 26 Jun 2024 11:33 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/89040 |
DOI: | 10.1093/plcell/koac176 |
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