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Searcey, Mark 
ORCID: https://orcid.org/0000-0003-2273-8949, McClean, Siobhán, Madden, Bernie and Wakelin, Laurence P. G.
(1997)
Bleomycin mimics. Design and synthesis of an acridine derivative which cleaves DNA in a sequence-neutral manner.
Journal of the Chemical Society. Perkin Transactions 2 (3).
pp. 523-532.
ISSN 0300-9580
Abstract
Bleomycin 1 is a clinically used anticancer drug that cleaves DNA at guanine-pyrimidine dinucleotides by a free-radical mechanism. The compound FTP1 2 was designed as a bleomycin mimic to cleave DNA in a non-sequence selective manner. Compound 2 may provide a route to novel, chemically simple, bleomycin analogues that also have potential as new DNA footprinting agents. FTP1 2 was synthesised from chelidamic acid, 1,3-phenylenediamine and 9-chloroacridine. An untargeted agent 8 was also made. Compound 2 cleaves DNA in a relatively non-sequence selective manner although a number of hotspots of similar sequence were found. It has a 20-fold higher cutting activity than the untargeted agent 8 and has been used to footprint two antibiotics, actinomycin D 9 and a minor groove binder, distamycin A 10. It gives clear, well-defined footprints which compare favourably with those reported by MPE-FeII, DNase I and iron-EDTA and is a useful addition to the range of footprinting agents. Its cytotoxicity against human colon carcinoma cells in culture is 20 times lower than that of bleomycin although its ability to cleave naked DNA is 10 000-fold less, suggesting that on a mole-for-mole basis FTP1-induced DNA damage is more biologically harmful.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | chemistry(all) ,/dk/atira/pure/subjectarea/asjc/1600 |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Oct 2022 12:32 |
| Last Modified: | 25 Oct 2022 00:15 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88819 |
| DOI: | 10.1039/a606218k |
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