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Bunk, Boyke, Schulz, Arne, Stammen, Simon, Münch, Richard, Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456, Rohde, Manfred, Jahn, Dieter and Biedendieck, Rebekka
(2010)
A short story about a big magic bug.
Bioengineered Bugs, 1 (2).
pp. 85-91.
ISSN 1949-1018
Abstract
Bacillus megaterium, the "big beast," is a Gram-positive bacterium with a size of 4 x 1.5 μm. During the last years, it became more and more popular in the field of biotechnology for its recombinant protein production capacity. For the purpose of intra- as well as extracellular protein synthesis several vectors were constructed and commercialized (MoBiTec GmbH, Germany). On the basis of two compatible vectors, a T7 RNA polymerase driven protein production system was established. Vectors for chromosomal integration enable the direct manipulation of the genome. The vitamin B 12 biosynthesis of B. megaterium served as a model for the systematic development of a production strain using these tools. For this purpose, the overexpression of chromosomal and plasmid encoded genes and operons, the synthesis of anti-sense RNA for gene silencing, the removal of inhibitory regulatory elements in combination with the utilization of strong promoters, directed protein design, and the recombinant production of B 12 binding proteins to overcome feedback inhibition were successfully employed. For further system biotechnology based optimization strategies the genome sequence will provide a closer look into genomic capacities of B. megaterium. DNA arrays are available. Proteome, fluxome and metabolome analyses are possible. All data can be integrated by using a novel bioinformatics platform. Finally, the size of the "big beast" B. megaterium invites for cell biology research projects. All these features provide a solid basis for challenging biotechnological approaches.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: We would like to thank Stefan Leupold for fluorescent images. We gratefully acknowledge financial support granted by the Deutsche Forschungsgemeinschaft, especially “Sonderforschungsbereich 578,” and by the “Fonds der Chemischen Industrie.” |
| Uncontrolled Keywords: | bacillus megaterium,protein secretion,recombinant protein production,vector system,vitamin b,biotechnology,bioengineering,applied microbiology and biotechnology ,/dk/atira/pure/subjectarea/asjc/1300/1305 |
| Faculty \ School: | Faculty of Science |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Sep 2022 15:30 |
| Last Modified: | 24 Oct 2022 16:33 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88522 |
| DOI: | 10.4161/bbug.1.2.11101 |
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