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Storbeck, Sonja, Rolfes, Sarah, Raux-Deery, Evelyne, Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456, Jahn, Dieter and Layer, Gunhild
(2010)
A novel pathway for the biosynthesis of heme in Archaea: Genome-based bioinformatic predictions and experimental evidence.
Archaea, 2010.
ISSN 1472-3646
Abstract
Heme is an essential prosthetic group for many proteins involved in fundamental biological processes in all three domains of life. In Eukaryota and Bacteria heme is formed via a conserved and well-studied biosynthetic pathway. Surprisingly, in Archaea heme biosynthesis proceeds via an alternative route which is poorly understood. In order to formulate a working hypothesis for this novel pathway, we searched 59 completely sequenced archaeal genomes for the presence of gene clusters consisting of established heme biosynthetic genes and colocalized conserved candidate genes. Within the majority of archaeal genomes it was possible to identify such heme biosynthesis gene clusters. From this analysis we have been able to identify several novel heme biosynthesis genes that are restricted to archaea. Intriguingly, several of the encoded proteins display similarity to enzymes involved in heme d1 biosynthesis. To initiate an experimental verification of our proposals two Methanosarcina barkeri proteins predicted to catalyze the initial steps of archaeal heme biosynthesis were recombinantly produced, purified, and their predicted enzymatic functions verified.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | microbiology,physiology,ecology, evolution, behavior and systematics ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Sep 2022 15:30 |
| Last Modified: | 19 Apr 2023 01:14 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88517 |
| DOI: | 10.1155/2010/175050 |
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