Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation

Juodeikis, Rokas, Lee, Matthew J., Mayer, Matthias, Mantell, Judith, Brown, Ian R., Verkade, Paul, Woolfson, Derek N., Prentice, Michael B., Frank, Stefanie and Warren, Martin J. ORCID: https://orcid.org/0000-0002-6028-6456 (2020) Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation. MicrobiologyOpen, 9 (5). ISSN 2045-8827

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Abstract

Metabolosomes, catabolic bacterial microcompartments (BMCs), are proteinaceous organelles that are associated with the breakdown of metabolites such as propanediol and ethanolamine. They are composed of an outer multicomponent protein shell that encases a specific metabolic pathway. Protein cargo found within BMCs is directed by the presence of an encapsulation peptide that appears to trigger aggregation before the formation of the outer shell. We investigated the effect of three distinct encapsulation peptides on foreign cargo in a recombinant BMC system. Our data demonstrate that these peptides cause variations in enzyme activity and protein aggregation. We observed that the level of protein aggregation generally correlates with the size of metabolosomes, while in the absence of cargo BMCs self-assemble into smaller compartments. The results agree with a flexible model for BMC formation based around the ability of the BMC shell to associate with an aggregate formed due to the interaction of encapsulation peptides.

Item Type: Article
Additional Information: Funding Information: We are grateful to the Biotechnology and Biological Sciences Research Council of the UK for a strategic LoLa Award to MJW, DNW, and PV (BB/M002969/1). Publisher Copyright: © 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
Uncontrolled Keywords: bacterial organelles,cargo,protein aggregation,synthetic biology,targeting,microbiology ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Science
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Depositing User: LivePure Connector
Date Deposited: 20 Sep 2022 14:30
Last Modified: 21 Sep 2022 00:22
URI: https://ueaeprints.uea.ac.uk/id/eprint/88477
DOI: 10.1002/mbo3.1010

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