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Juodeikis, Rokas, Lee, Matthew J., Mayer, Matthias, Mantell, Judith, Brown, Ian R., Verkade, Paul, Woolfson, Derek N., Prentice, Michael B., Frank, Stefanie and Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456
(2020)
Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation.
MicrobiologyOpen, 9 (5).
ISSN 2045-8827
Abstract
Metabolosomes, catabolic bacterial microcompartments (BMCs), are proteinaceous organelles that are associated with the breakdown of metabolites such as propanediol and ethanolamine. They are composed of an outer multicomponent protein shell that encases a specific metabolic pathway. Protein cargo found within BMCs is directed by the presence of an encapsulation peptide that appears to trigger aggregation before the formation of the outer shell. We investigated the effect of three distinct encapsulation peptides on foreign cargo in a recombinant BMC system. Our data demonstrate that these peptides cause variations in enzyme activity and protein aggregation. We observed that the level of protein aggregation generally correlates with the size of metabolosomes, while in the absence of cargo BMCs self-assemble into smaller compartments. The results agree with a flexible model for BMC formation based around the ability of the BMC shell to associate with an aggregate formed due to the interaction of encapsulation peptides.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Biotechnology and Biological Sciences Research Council of the UK for a strategic LoLa Award to MJW, DNW, and PV (BB/M002969/1). |
| Uncontrolled Keywords: | bacterial organelles,cargo,protein aggregation,synthetic biology,targeting,microbiology ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Sep 2022 14:30 |
| Last Modified: | 07 Jun 2024 12:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88477 |
| DOI: | 10.1002/mbo3.1010 |
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