Biosynthesis of cobamides:Methods for the detection, analysis and production of cobamides and biosynthetic intermediates

Deery, Evelyne, Lawrence, Andrew D. and Warren, Martin J. ORCID: https://orcid.org/0000-0002-6028-6456 (2022) Biosynthesis of cobamides:Methods for the detection, analysis and production of cobamides and biosynthetic intermediates. In: Coenzyme B Enzymes Part A. Methods in Enzymology . Academic Press Inc., pp. 3-23. ISBN 9780323915915

Full text not available from this repository. (Request a copy)

Abstract

Vitamin B12, cobalamin, belongs to the broader cobamide family whose members are characterized by the presence of a cobalt-containing corrinoid ring. The ability to detect, isolate and characterize cobamides and their biosynthetic intermediates is an important prerequisite when attempting to study the synthesis of this remarkable group of compounds that play diverse roles across the three kingdoms of life. The synthesis of cobamides is restricted to only certain prokaryotes and their structural complexity entails an equally complex synthesis orchestrated through a multi-step biochemical pathway. In this chapter, we have outlined methods that we have found extremely helpful in the characterization of the biochemical pathway, including a plate microbiological assay, a corrinoid affinity extraction method, LCMS characterization and a multigene cloning strategy.

Item Type: Book Section
Additional Information: Publisher Copyright: © 2022 Elsevier Inc.
Uncontrolled Keywords: biosynthesis,cobalamin,cobalt,corrinoid,pathway,tetrapyrrole,vitamin b,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 20 Sep 2022 14:30
Last Modified: 27 Sep 2022 23:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/88469
DOI: 10.1016/bs.mie.2022.01.013

Actions (login required)

View Item View Item