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Deery, Evelyne, Lawrence, Andrew D. and Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456
(2022)
Biosynthesis of cobamides:Methods for the detection, analysis and production of cobamides and biosynthetic intermediates.
In:
Coenzyme B Enzymes Part A.
Methods in Enzymology
.
Academic Press Inc., pp. 3-23.
ISBN 9780323915915
Abstract
Vitamin B12, cobalamin, belongs to the broader cobamide family whose members are characterized by the presence of a cobalt-containing corrinoid ring. The ability to detect, isolate and characterize cobamides and their biosynthetic intermediates is an important prerequisite when attempting to study the synthesis of this remarkable group of compounds that play diverse roles across the three kingdoms of life. The synthesis of cobamides is restricted to only certain prokaryotes and their structural complexity entails an equally complex synthesis orchestrated through a multi-step biochemical pathway. In this chapter, we have outlined methods that we have found extremely helpful in the characterization of the biochemical pathway, including a plate microbiological assay, a corrinoid affinity extraction method, LCMS characterization and a multigene cloning strategy.
| Item Type: | Book Section |
|---|---|
| Additional Information: | Publisher Copyright: © 2022 Elsevier Inc. |
| Uncontrolled Keywords: | biosynthesis,cobalamin,cobalt,corrinoid,pathway,tetrapyrrole,vitamin b,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Sep 2022 14:30 |
| Last Modified: | 19 Apr 2023 01:29 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88469 |
| DOI: | 10.1016/bs.mie.2022.01.013 |
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