Comparative examination of Escherichia coli O157:H7 survival on romaine lettuce and in soil at two independent experimental sites

Bezanson, Greg, Delaquis, Pascal, Bach, Susan, McKellar, Robin, Topp, Ed, Gill, Alex, Blais, Burton and Gilmour, Matthew (2012) Comparative examination of Escherichia coli O157:H7 survival on romaine lettuce and in soil at two independent experimental sites. Journal of Food Protection, 75 (3). pp. 480-487. ISSN 0362-028X

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Abstract

Little is known about the influence of abiotic factors such as climate and soil chemistry on the survival of Escherichia coli O157:H7 in field lettuce. We applied a nalidixic acid-resistant derivative of strain ATCC 700728 to field-grown romaine lettuce in two regions in Canada characterized by large variances in soil type and climate. Surviving populations in soil and on lettuce leaves were estimated on sorbitol MacConkey agar supplemented with nalidixic acid. Data were fitted with the Weibull decline function to permit comparison of decay rates in the two experimental sites. E. coli O157:H7 populations fell from 10 5 to <10 2 CFU/g on leaves, and <10 3 CFU/g in soil within 7 days after inoculation. Analysis revealed there was no significant difference between decay rates at the two experimental sites in either environment. The results of this study suggest that the inherent ecological fitness of E. coli O157:H7 ATCC 700728 determines the extent of survival in the production environment.

Item Type:	Article
Uncontrolled Keywords:	food science,microbiology,sdg 13 - climate action ,/dk/atira/pure/subjectarea/asjc/1100/1106
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	14 Sep 2022 09:31
Last Modified:	21 Oct 2022 01:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/88247
DOI:	10.4315/0362-028X.JFP-11-306

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