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ToolsTran, Thien Toan, Clark, Kelley, Ma, Wenbo and Mulchandani, Ashok (2020) Detection of a secreted protein biomarker for citrus Huanglongbing using a single-walled carbon nanotubes-based chemiresistive biosensor. Biosensors and Bioelectronics, 147. ISSN 0956-5663
Full text not available from this repository. (Request a copy)Abstract
Citrus greening, or Huanglongbing (HLB), is currently the most devasting disease of citrus, creating unprecedented crisis for the multibillion-dollar global citrus industry. To-date, there is no effective cure and disease management relies on early detection and removal of infected trees. Thus, it is imperative that accurate, timely, and robust disease detection and diagnosis technologies are available to minimize the spread of disease. This study reports a sensitive and selective label-free biosensor that combines the physical and chemical advantages of carbon nanomaterials like single-walled carbon nanotubes (SWNTs) in a field-effect transistor (FET)/chemiresistor architecture with selective antibodies against Sec-delivered effector 1 (SDE1), a secreted protein biomarker, for the detection of HLB. The biosensor detected SDE1 biomarkers for citrus greening in plant tissue extracts with the dynamic range over three orders of magnitude in the low nanomolar to micromolar concentration range and limit of detection of 5 nM. The study also demonstrated the use of the standard additions assay method with the biosensor to attain a 90-percent signal recovery in concentrated plant tissue extract, allowing for quantitative detection without an external calibration. Adopting the novel detection strategy targeting the secreted protein biomarker, SDE1, addresses some of the challenges faced by current methods of nucleic acid-based assays and symptom-based diagnosis, which have been found prone to false negatives and misdiagnoses, respectively.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by grants from U.S. Department of Agriculture (2014-67021-21589) to AM and WM and National Science Foundation (1606181 and 1842718) to AM. AM acknowledges the W. Ruel Johnson Chair in Environmental Engineering. |
| Uncontrolled Keywords: | candidatus liberibacter asiaticus,chemiresistor,citrus huanglongbing,secretory biomarker,single-walled carbon nanotubes,standard addition method,biotechnology,biophysics,biomedical engineering,electrochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1305 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 12 Sep 2022 12:30 |
| Last Modified: | 25 Sep 2024 16:45 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/88143 |
| DOI: | 10.1016/j.bios.2019.111766 |
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