Effectiveness of glass beads for plating cell cultures

Prusokas, Alidivinas, Hawkins, Michelle, Nieduszynski, Conrad A. ORCID: https://orcid.org/0000-0003-2001-076X and Retkute, Renata (2021) Effectiveness of glass beads for plating cell cultures. Physical Review E, 103 (5). ISSN 2470-0045

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Abstract

Cell plating, the spreading out of a liquid suspension of cells on a surface followed by colony growth, is a common laboratory procedure in microbiology. Despite this, the exact impact of its parameters on colony growth has not been extensively studied. A common protocol involves the shaking of glass beads within a Petri dish containing solid growth media. We investigated the effects of multiple parameters in this protocol: the number of beads, the shape of movement, and the number of movements. Standard suspensions of Escherichia coli were spread while varying these parameters to assess their impact on colony growth. Results were assessed by a variety of metrics: the number of colonies, the mean distance between closest colonies, and the variability and uniformity of their spatial distribution. Finally, we devised a mathematical model of shifting billiard to explain the heterogeneities in the observed spatial patterns. Exploring the parameters that affect the most fundamental techniques in microbiology allows us to better understand their function, giving us the ability to precisely control their outputs for our exact needs.

Item Type: Article
Additional Information: Publisher Copyright: © 2021 American Physical Society.
Uncontrolled Keywords: statistical and nonlinear physics,statistics and probability,condensed matter physics ,/dk/atira/pure/subjectarea/asjc/3100/3109
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 08 Sep 2022 10:30
Last Modified: 04 Oct 2022 00:19
URI: https://ueaeprints.uea.ac.uk/id/eprint/87933
DOI: 10.1103/PhysRevE.103.052410

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