Cohesin-mediated genome architecture does not define DNA replication timing domains

Oldach, Phoebe and Nieduszynski, Conrad A. ORCID: (2019) Cohesin-mediated genome architecture does not define DNA replication timing domains. Genes, 10 (3). ISSN 2073-4425

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3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.

Item Type: Article
Additional Information: Funding Information: Funding: This work was supported by Biotechnology and Biological Sciences Research Council grants BB/N016858/1 and Wellcome Trust Investigator Award 110064/Z/15/Z. PFO is generously supported by a Wellcome Trust studentship award 203727/Z/16/Z.
Uncontrolled Keywords: chromatin interactions,cohesin,dna,genome architecture,replication timing,genetics,genetics(clinical) ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 07 Sep 2022 14:31
Last Modified: 21 Oct 2022 01:37
DOI: 10.3390/genes10030196

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