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Oldach, Phoebe and Nieduszynski, Conrad A. 
ORCID: https://orcid.org/0000-0003-2001-076X
(2019)
Cohesin-mediated genome architecture does not define DNA replication timing domains.
Genes, 10 (3).
ISSN 2073-4425
Abstract
3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Funding: This work was supported by Biotechnology and Biological Sciences Research Council grants BB/N016858/1 and Wellcome Trust Investigator Award 110064/Z/15/Z. PFO is generously supported by a Wellcome Trust studentship award 203727/Z/16/Z. |
| Uncontrolled Keywords: | chromatin interactions,cohesin,dna,genome architecture,replication timing,genetics,genetics(clinical) ,/dk/atira/pure/subjectarea/asjc/1300/1311 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 07 Sep 2022 14:31 |
| Last Modified: | 21 Oct 2022 01:37 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/87855 |
| DOI: | 10.3390/genes10030196 |
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