Swainsbury, David J. K., Scheidelaar, Stefan, van Grondelle, Rienk, Killian, J. Antoinette and Jones, Michael R. (2014) Bacterial reaction centers purified with styrene maleic acid copolymer retain native membrane functional properties and display enhanced stability. Angewandte Chemie - International Edition, 53 (44). pp. 11803-11807. ISSN 1433-7851
Full text not available from this repository. (Request a copy)Abstract
Integral membrane proteins often present daunting challenges for biophysical characterization, a fundamental issue being how to select a surfactant that will optimally preserve the individual structure and functional properties of a given membrane protein. Bacterial reaction centers offer a rare opportunity to compare the properties of an integral membrane protein in different artificial lipid/surfactant environments with those in the native bilayer. Here, we demonstrate that reaction centers purified using a styrene maleic acid copolymer remain associated with a complement of native lipids and do not display the modified functional properties that typically result from detergent solubilization. Direct comparisons show that reaction centers are more stable in this copolymer/lipid environment than in a detergent micelle or even in the native membrane, suggesting a promising new route to exploitation of such photovoltaic integral membrane proteins in device applications.
Item Type: | Article |
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Additional Information: | Publisher Copyright: © 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Uncontrolled Keywords: | detergents,membrane proteins,nanodiscs,reaction centers,styrene maleic acid,catalysis,chemistry(all),sdg 7 - affordable and clean energy ,/dk/atira/pure/subjectarea/asjc/1500/1503 |
Faculty \ School: | Faculty of Science > School of Biological Sciences |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 17 Aug 2022 12:32 |
Last Modified: | 22 Oct 2022 07:55 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/87374 |
DOI: | 10.1002/anie.201406412 |
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