Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea

Kelemen, Gabriella H., Cundliffe, Eric and Financsek, István (1991) Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea. Gene, 98 (1). pp. 53-60. ISSN 0378-1119

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Abstract

Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.

Item Type: Article
Additional Information: Funding Information: Part of the study was supported by a post-doctoral fellowship of the Leverhulme Trust awarded to G.H.K.
Uncontrolled Keywords: aminoglycoside-resistance,recombinant dna,ribosomal rna methylase,transcript mapping,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 19 Jul 2022 16:30
Last Modified: 20 Jul 2022 00:25
URI: https://ueaeprints.uea.ac.uk/id/eprint/86645
DOI: 10.1016/0378-1119(91)90103-I

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