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ToolsKelemen, Gabriella H., Cundliffe, Eric and Financsek, István (1991) Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea. Gene, 98 (1). pp. 53-60. ISSN 0378-1119
Full text not available from this repository. (Request a copy)Abstract
Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: Part of the study was supported by a post-doctoral fellowship of the Leverhulme Trust awarded to G.H.K. |
| Uncontrolled Keywords: | aminoglycoside-resistance,recombinant dna,ribosomal rna methylase,transcript mapping,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1311 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 19 Jul 2022 16:30 |
| Last Modified: | 23 Oct 2022 04:04 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/86645 |
| DOI: | 10.1016/0378-1119(91)90103-I |
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