Transcriptional attenuation control of the tylosin‐resistance gene tlrA in Streptomyces fradiae

Kelemen, Gabriella H., Zalacain, Magdalena, Culebras, Esther, Seno, Eugene T. and Cundliffe, Eric (1994) Transcriptional attenuation control of the tylosin‐resistance gene tlrA in Streptomyces fradiae. Molecular Microbiology, 14 (4). pp. 833-842. ISSN 0950-382X

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Abstract

The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase‐coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome‐mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.

Item Type: Article
Uncontrolled Keywords: microbiology,molecular biology ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 19 Jul 2022 16:30
Last Modified: 04 Aug 2022 16:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/86644
DOI: 10.1111/j.1365-2958.1994.tb01319.x

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