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Cooper, C. S. 
ORCID: https://orcid.org/0000-0003-2013-8042, Tempest, P. R., Beckman, M. P., Heldin, C. H. and Brookes, P.
(1986)
Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts.
The EMBO Journal, 5 (10).
pp. 2623-2628.
ISSN 0261-4189
Abstract
We have identified a class of transformed NIH3T3 mouse fibroblasts that arise at low frequencies in transfection experiments with DNA from both neoplastic and non-neoplastic cells and that may result from a low level of spontaneous transformation of NIH3T3 cells. DNA from the transformed cells was unable to transform NIH3T3 cells in a second cycle of transfection and, where examined, the cells showed no evidence for the uptake of the transfected DNA sequences. The results of Southern analyses demonstrate that a mouse homologue of the human met oncogene is amplified 4- to 8-fold in 7 of 10 lines of these transformed NIH3T3 mouse fibroblasts. The cells containing the amplified gene also exhibit at least a 20-fold overexpression of an 8.5-kb mRNA that is homologous to met. To test the hypothesis that met encodes a growth factor receptor, we examined the binding of platelet-derived growth factor, epidermal growth factor, insulin-like growth factor I and gastrin-releasing peptide to transformed and non-transformed NIH3T3 cells. The results show that there is no significant elevation of the binding of these growth factors to cells containing amplification and overexpression of met.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | neuroscience(all),molecular biology,biochemistry, genetics and molecular biology(all),immunology and microbiology(all) ,/dk/atira/pure/subjectarea/asjc/2800 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Jul 2022 17:30 |
| Last Modified: | 23 Oct 2022 04:02 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/86542 |
| DOI: | 10.1002/j.1460-2075.1986.tb04543.x |
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