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Kiernan, B. W., Garcion, E., Ferguson, J., Frost, E. E., Torres, E. M., Dunnett, S. B., Saga, Y., Aizawa, S., Faissner, A., Kaur, R., Franklin, R. J.M. and Ffrench-Constant, C. 
ORCID: https://orcid.org/0000-0002-5621-3377
(2001)
Myelination and behaviour of tenascin-C null transgenic mice.
European Journal of Neuroscience, 11 (9).
pp. 3082-3092.
ISSN 0953-816X
Abstract
The extracellular matrix glycoprotein tenascin-C is widely expressed during development and repair, making it surprising that few abnormalities have been found in transgenic mice lacking this molecule. We have therefore re-examined the transgenic mice described by Saga et al. in which tenascin-C was knocked-out by homologous recombination, focusing on two aspects of the nervous system likely to reveal any abnormalities that might follow the loss of tenascin-C. First, we have determined the pattern of myelin and distribution of oligodendrocyte precursor cells in those areas, such as the optic nerve and retina where local concentrations of tenascin-C have been proposed to act as barriers to oligodendrocyte precursor migration and so prevent inappropriate myelination. Secondly, we have examined the behaviour of the mice in a number of well-characterized tests, e.g. beam-walking, passive avoidance and the Morris water maze. We find no abnormalities of myelination or oligodendrocyte precursor distribution in adult mice, showing that local concentrations of tenascin-C are not the sole mechanism responsible for the pattern of myelination in these regions of CNS. However, we do find a number of behavioural abnormalities in these mice and show that hyperlocomotion and deficits in coordination during beam walking can be ascribed to tenascin-C deficiency. The effects on coordination are, however, not seen on a 129 genetic background. Taken together, these results significantly extend the phenotype associated with tenascin-C deficiency but argue against a role in myelination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | hyperlocomotion,lamina cribrosa,migration,morris water maze,oligodendrocyte precursor,passive avoidance,neuroscience(all) ,/dk/atira/pure/subjectarea/asjc/2800 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 16 Jul 2022 13:30 |
| Last Modified: | 23 Oct 2022 03:58 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/86373 |
| DOI: | 10.1046/j.1460-9568.1999.00729.x |
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