Generation of an environmental niche for neural stem cell development by the extracellular matrix molecular tenascin C

Garcion, Emmanuel, Halilagic, Aida, Faissner, Andreas and ffrench-Constant, Charles ORCID: https://orcid.org/0000-0002-5621-3377 (2004) Generation of an environmental niche for neural stem cell development by the extracellular matrix molecular tenascin C. Development, 131 (14). pp. 3423-3432. ISSN 0950-1991

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Abstract

Stem cells in the embryonic mammalian CNS are initially responsive to fibroblast growth factor 2 (FGF2). They then undergo a developmental programme in which they acquire epidermal growth factor (EGF) responsiveness, switch from the production of neuronal to glial precursors and become localized in specialized germinal zones such as the subventricular zone (SVZ). Here we show that extracellular matrix molecules act as regulators of this programme. Tenascin C is highly expressed in the SVZ, and transgenic mice lacking tenascin C show delayed acquisition of the EGF receptor. This results from alterations in the response of the stem cells to the growth factors FGF2 and bone morphogenic protein 4 (BMP4), which normally promote and inhibit acquisition of the EGF receptor, respectively. Tenascin C-deficient mice also have altered numbers of CNS stem cells and these stem cells have an increased probability of generating neurones when grown in cell culture. We conclude that tenascin C contributes to the generation of a stem cell 'niche' within the SVZ, acting to orchestrate growth factor signalling so as to accelerate neural stem cell development.

Item Type:	Article
Uncontrolled Keywords:	central nervous system,differentiation,gliogenesis,growth factor,neurogenesis,neurosphere,proliferation,stem cell,tenascin c,molecular biology,developmental biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	16 Jul 2022 11:31
Last Modified:	25 Sep 2024 16:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/86336
DOI:	10.1242/dev.01202

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