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McEwan, Alastair G., Richardson, David J. 
ORCID: https://orcid.org/0000-0002-6847-1832, Hudig, Hendrik, Ferguson, Stuart J. and Jackson, J. Barry
(1989)
Identification of cytochromes involved in electron transport to trimethylamine N-oxide/dimethylsulphoxide reductase in Rhodobacter capsulatus.
BBA - Bioenergetics, 973 (2).
pp. 308-314.
ISSN 0005-2728
Abstract
The role of cytochromes in the electron-transport pathway to trimethylamine N-oxide (TMAO)/dimethylsulphoxide (DMSO) reductase in the photosynthetic bacterium Rhodobacter capsulatus was investigated. Reduced-minus-oxidized difference spectra in intact cells with TMAO or DMSO as oxidant revealed cytochrome absorbance changes with a maximum at 559 nm and a shoulder between 548 nm and 556 nm. The former change indicates a role for a 6-type cytochrome and the latter for a c-type cytochrome, both of which are distinct from the cytochrome bc1 complex. Cytochrome c-556 was identified in a bacterial periplasmic fraction as a redox component which couldbe oxidised by TMAO or DMSO. Cytochrome c-556 was the only cytochrome species which co-fractionated with TMAO/DMSO reductase following gel filtration of a post-chromatophore supernatant produced after French presstreatment of intact cells. The mid-point redox potential (pH 7.6) of cytochrome c-556 was + 105 mV (n = 1). It is suggested that TMAO/DMSO reductase and cytochrome c-556 form a structural and functional association in the periplasm of Rhodobacter capsulatus.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: This work was supported by the SERC (U.K.) via grants to S.J.F. and J.B.J. and a studentship to D.J.R. |
| Uncontrolled Keywords: | (rb. capsulatus),cytochrome c-556,electron transport,periplasmic protein,photosynthetic bacterium,dmso reductase,biophysics,biochemistry,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 15 Jul 2022 13:30 |
| Last Modified: | 15 May 2023 00:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/86222 |
| DOI: | 10.1016/S0005-2728(89)80437-2 |
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