The purification of a cd1‐type nitrite reductase from, and the absence of a copper‐type nitrite reductase from, the aerobic denitrifier Thiosphaera pantotropha; the role of pseudoazurin as an electron donor

Moir, James W. B., Baratta, Daniela, Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Ferguson, Stuart J. (1993) The purification of a cd1‐type nitrite reductase from, and the absence of a copper‐type nitrite reductase from, the aerobic denitrifier Thiosphaera pantotropha; the role of pseudoazurin as an electron donor. European Journal of Biochemistry, 212 (2). pp. 377-385. ISSN 0014-2956

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Abstract

Thiosphaera pantotropha has been reported to contain a copper‐type nitrite reductase on the basis that the copper chelator diethyldithiocarbamate inhibited the overall process of denitrification. It is now shown that nitrous oxide reduction is 100% inhibited by 10 mM diethyldithiocarbamate or 100 μM azide. We also found that both these inhibitors partially inhibited nitrite reduction in this organism. We purified the nitrite reductase of T. pantotropha and found that it was of the cytochrome cd1 type, contrary to the published report of it being a copper‐type nitrite reductase. This is of importance since T. pantotropha is capable of acrobic nitrite reduction. The only detectable nitrite reduction in anaerobically or aerobically grown cells is the cd1 type. We also purified a small copper‐containing protein, pseudoazurin. Pseudoazurin was found to be capable of donating electrons to the cd1‐type nitrite reductase in vitro, and its copper centre was chelated by diethyldithiocarbamate. Since nitrite reduction is partially inhibited by diethyldithiocar‐bamate, it is thought that pseudoazurin is an electron donor to nitrite reductase in vivo.

Item Type:	Article
Uncontrolled Keywords:	biochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	15 Jul 2022 13:30
Last Modified:	15 May 2023 00:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/86213
DOI:	10.1111/j.1432-1033.1993.tb17672.x

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