Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase

Breton, Jacques, Berks, Ben C., Reilly, Ann, Thomson, Andrew J., Ferguson, Stuart J. and Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 (1994) Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase. FEBS Letters, 345 (1). pp. 76-80. ISSN 0014-5793

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Abstract

Electron paramagnetic resonance spectroscopy signals attributable to low-spin haem c in the oxidised protein and [4Fe4S]1+ in the dithionite-reduced protein were identified, at low temperature, in Thiosphaera pantotropha periplasmic nitrate reductase. Spin integration of these signals as well as elemental analysis suggest a stoichiometry of 1.3-1.6 c-haem and 1 [4Fe4S] cluster per enzyme molecule. The Em (at pH 7.4) of the [4F4S]2+,1+ couple, -160 mV, means that it is unlikely to be physiologically reducible. Peptide sequences from the 90 kDa subunit indicate that the enzyme is a member of the family of molybdopterin guanine dinucleotide-binding polypeptides, the majority of which possess a putative [4Fe4S] cluster binding sequence and thus may also bind a (low potential) iron-sulphur cluster.

Item Type: Article
Additional Information: Funding Information: sequences. This research was supported by SERUAFRC through a TRAMPS award to D.J.R. and S.J.F. (GR/HJ2225). A.J.T. and D.J.R. thank the SERC for support provided to the Centre for Metalloprotein Spectroscopy and Biology (GR/JOO786).
Uncontrolled Keywords: cytochrome c-type,iron-sulphur protein,periplasmic nitrate reductase,thiosphaera pantotropha,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School:
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 15 Jul 2022 12:31
Last Modified: 13 Aug 2022 02:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/86206
DOI: 10.1016/0014-5793(94)00445-5

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