Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase

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Breton, Jacques, Berks, Ben C., Reilly, Ann, Thomson, Andrew J., Ferguson, Stuart J. and Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 (1994) Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase. FEBS Letters, 345 (1). pp. 76-80. ISSN 0014-5793

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Abstract

Electron paramagnetic resonance spectroscopy signals attributable to low-spin haem c in the oxidised protein and [4Fe4S]1+ in the dithionite-reduced protein were identified, at low temperature, in Thiosphaera pantotropha periplasmic nitrate reductase. Spin integration of these signals as well as elemental analysis suggest a stoichiometry of 1.3-1.6 c-haem and 1 [4Fe4S] cluster per enzyme molecule. The Em (at pH 7.4) of the [4F4S]2+,1+ couple, -160 mV, means that it is unlikely to be physiologically reducible. Peptide sequences from the 90 kDa subunit indicate that the enzyme is a member of the family of molybdopterin guanine dinucleotide-binding polypeptides, the majority of which possess a putative [4Fe4S] cluster binding sequence and thus may also bind a (low potential) iron-sulphur cluster.

Item Type:	Article
Additional Information:	Funding Information: sequences. This research was supported by SERUAFRC through a TRAMPS award to D.J.R. and S.J.F. (GR/HJ2225). A.J.T. and D.J.R. thank the SERC for support provided to the Centre for Metalloprotein Spectroscopy and Biology (GR/JOO786).
Uncontrolled Keywords:	cytochrome c-type,iron-sulphur protein,periplasmic nitrate reductase,thiosphaera pantotropha,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	15 Jul 2022 12:31
Last Modified:	15 May 2023 00:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/86206
DOI:	10.1016/0014-5793(94)00445-5

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