Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans

Sears, H. J., Bennett, B., Spiro, S., Thomson, A. J. and Richardson, D. J. ORCID: https://orcid.org/0000-0002-6847-1832 (1995) Identification of periplasmic nitrate reductase Mo(V) EPR signals in intact cells of Paracoccus denitrificans. Biochemical Journal, 310 (1). pp. 311-314. ISSN 0264-6021

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Abstract

EPR spectroscopy has been successfully used to detect signals due to molybdenum (V) and ferric iron in intact cells of aerobically grown Paracoccus denitrificans. The signals are ascribed to the catalytic molybdenum centre and to the haem iron of the periplasmic nitrate reductase. These signals are absent from a mutant strain deficient in this enzyme. The Mo(V) signal is due to the High-g Split species which has been well characterized in the purified enzyme. This confirms that the High-g Split is the physiologically relevant signal of a number observed in the previous work on the purified enzyme.

Item Type:	Article
Uncontrolled Keywords:	biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	15 Jul 2022 12:30
Last Modified:	15 May 2023 00:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/86200
DOI:	10.1042/bj3100311

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