Purification of hydroxylamine oxidase from Thiosphaera pantotropha. Identification of electron acceptors that couple heterotrophic nitrification to aerobic denitrification

Wehrfritz, Josa Marie, Reilly, Ann, Spiro, Stephen and Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 (1993) Purification of hydroxylamine oxidase from Thiosphaera pantotropha. Identification of electron acceptors that couple heterotrophic nitrification to aerobic denitrification. FEBS Letters, 335 (2). pp. 246-250. ISSN 0014-5793

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Abstract

Thiosphaera pantotropha, a Gram-negative heterotrophic nitrifying bacterium, expresses a soluble 20 kDa monomeric periplasmic hydroxylamine oxidase that differs markedly from the hydroxylamine oxidase found in autotrophic bacteria. This enzyme can use the periplasmic redox proteins, cytochrome C551 and pseudoazurin as electron acceptors, both of which can also donate electrons to denitrification enzymes. A model of electron transfer is proposed, that suggests a coupling of nitrification to denitrification and provides a mechanism by which nitrification can play a role in dissipating reductant.

Item Type:	Article
Uncontrolled Keywords:	denitrification,hydroxylamine oxidase,nitrification,thiosphaera pantotropha,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	14 Jul 2022 17:30
Last Modified:	15 May 2023 00:54
URI:	https://ueaeprints.uea.ac.uk/id/eprint/86153
DOI:	10.1016/0014-5793(93)80739-H

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