c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE (Journal of Biological Chemistry (2010) 285, (22882-22889))

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Goddard, Alan D., Stevens, Julie M., Rao, Feng, Mavridou, Despoina A.I., Chan, Weelee, Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, Allen, James W.A. and Ferguson, Stuart J. (2011) c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE (Journal of Biological Chemistry (2010) 285, (22882-22889)). Journal of Biological Chemistry, 286 (33). p. 29442. ISSN 0021-9258

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Abstract

The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

Item Type: Article
Uncontrolled Keywords: biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
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Depositing User: LivePure Connector
Date Deposited: 14 Jul 2022 16:30
Last Modified: 15 May 2023 00:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/86118
DOI: 10.1074/jbc.A110.133421

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