Biochemistry and Molecular Biology of Nitrification

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Ferguson, Stuart J., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Van Spanning, Rob J.M. (2007) Biochemistry and Molecular Biology of Nitrification. In: Biology of the Nitrogen Cycle. Elsevier, pp. 209-222. ISBN 9780444528575

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Abstract

The biochemistry and molecular biology of nitrification are poorly understood, almost certainly related to the difficult problem of growing large enough quantities of cells from which to prepare vesicular membranes and purified proteins. This chapter explains the biochemistry and molecular biology of nitrification. Nitrosomonas and Nitrobacter depend on a chemiosmotic mechanism of energy transduction. Many of the special biochemical features of Nitrosomonas and Nitrobacter need to be understood in the context of the ability of the electron transport system to catalyze reversed electron transfer. The demonstration of H_ pumping by intact cells fed with electrons from the nonphysiological donor ascorbate can be taken as support for the H_ pumping activity. The genome sequence clearly shows two reading frames, designated NorA and NorB on the basis of earlier partial sequence information. Bioenergetic arguments have suggested a location at the cytoplasmic surface, but immunolabeling studies have indicated the opposite. The oxidation of ammonia to NO2- by Nitrosomonas is not a straightforward process. The idea that ubiquinol provides electrons for the ammonia mono-oxygenase is supported by the fact that partially purified preparations of the enzyme can use duroquinol as electron donor.

Item Type: Book Section
Uncontrolled Keywords: agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/1100
Faculty \ School:
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
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Depositing User: LivePure Connector
Date Deposited: 14 Jul 2022 16:30
Last Modified: 15 May 2023 01:10
URI: https://ueaeprints.uea.ac.uk/id/eprint/86115
DOI: 10.1016/B978-044452857-5.50015-1

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