Regulation of immune receptor kinase plasma membrane nanoscale organization by a plant peptide hormone and its receptors

Gronnier, Julien, Franck, Christina M., Stegmann, Martin, Defalco, Thomas A., Abarca, Alicia, von Arx, Michelle, Dünser, Kai, Lin, Wenwei, Yang, Zhenbiao, Kleine-Vehn, Jürgen, Ringli, Christoph and Zipfel, Cyril (2022) Regulation of immune receptor kinase plasma membrane nanoscale organization by a plant peptide hormone and its receptors. eLife, 11. ISSN 2050-084X

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Spatial partitioning is a propensity of biological systems orchestrating cell activities in space and time. The dynamic regulation of plasma membrane nano-environments has recently emerged as a key fundamental aspect of plant signaling, but the molecular components governing it are still mostly unclear. The receptor kinase FERONIA (FER) controls ligand-induced complex formation of the immune receptor kinase FLAGELLIN SENSING 2 (FLS2) with its co-receptor BRASSINOSTEROID-INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1), and perception of the endogenous peptide hormone RAPID ALKALANIZATION FACTOR 23 (RALF23) by FER inhibits immunity. Here, we show that FER regulates the plasma membrane nanoscale organization of FLS2 and BAK1. Our study demonstrates that akin to FER, leucine-rich repeat (LRR) extensin proteins (LRXs) contribute to RALF23 responsiveness and regulate BAK1 nanoscale organization and immune signaling. Furthermore, RALF23 perception leads to rapid modification of FLS2 and BAK1 nanoscale organization, and its inhibitory activity on immune signaling relies on FER kinase activity. Our results suggest that perception of RALF peptides by FER and LRXs actively modulates plasma membrane nanoscale organization to regulate cell surface signaling by other ligand-binding receptor kinases.

Item Type: Article
Faculty \ School: Faculty of Science > The Sainsbury Laboratory
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
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Depositing User: LivePure Connector
Date Deposited: 17 Feb 2022 15:30
Last Modified: 22 Oct 2022 17:35
DOI: 10.7554/eLife.74162


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